WebRecombinant Human Dihydrofolate Reductase Protein expressed from Escherichia coli (E. coli). Conjugated to His tag.Order Protein ABIN3079081 online. English +1 877 302 … WebSep 1, 2003 · Lane M.- Molecular weight marker (Gibco BRL 100bp ladder). Lane 1 ... alteration in chromosomal dihydrofolate reductase and the plasmid-encoded production of an additional dihydrofolate reductase ...

Pralatrexate (NSC 754230) ≥99%(HPLC) Selleck DHFR inhibitor

Dihydrofolate reductase, or DHFR, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as an electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. In humans, the DHFR enzyme is encoded by the DHFR gene. It is … See more Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a proton shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate … See more A central eight-stranded beta-pleated sheet makes up the main feature of the polypeptide backbone folding of DHFR. Seven of these strands are parallel and the eighth runs antiparallel. Four alpha helices connect successive beta strands. Residues 9 – 24 … See more DHFR is an attractive pharmaceutical target for inhibition due to its pivotal role in DNA precursor (thymine) synthesis. Trimethoprim, … See more Dihydrofolate reductase has been shown to interact with GroEL and Mdm2. See more General mechanism DHFR catalyzes the transfer of a hydride from NADPH to dihydrofolate with an accompanying … See more DHFR mutations cause dihydrofolate reductase deficiency, a rare autosomal recessive inborn error of folate metabolism that results in See more DHFR has been used as a tool to detect protein–protein interactions in a protein-fragment complementation assay (PCA), using a split-protein approach. DHFR-lacking CHO cells are the most commonly used cell line for the production of recombinant … See more WebFind dihydrofolate reductase and related products for scientific research at MilliporeSigma. US EN. Applications Products Services Support. Advanced Search. Structure Search. ... Molecular Weight: 454.44 (anhydrous basis) Compare Product No. Description SDS Pricing; A6770: ≥98% (HPLC), powder: Expand. Hide. corsair commander pro argb

Dihydrofolate Reductase - an overview ScienceDirect …

WebNov 23, 1999 · Dihydrofolate reductase (DHFR) catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate and is essential for the synthesis of thymidylate, purines and several amino acids. Inhibition of the enzyme's activity leads to arrest of DNA synthesis and cell death. WebFeb 1, 1973 · Bovine hepatic dihydrofolate reductase has been purified to homogeneity by traditional methods of enzyme purification. The enzyme of molecular weight 20,000 exhibited a classical double pH optimum ... WebAntibody to the purified enzyme has been used to study its synthesis and intracellular turnover. The antibody specifically precipitates one protein from T4D-infected cell … braxton pool