Fth1 abcam
WebThe FTH1 Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to FTH1. This antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The FTH1 Antibody has been validated for the following applications: Western Blot. FTH1 Antibody detects endogenous levels of total FTH1 protein. Nonspecific bands are seen above 80 ... WebFeb 2, 2024 · Ferritin is a ubiquitously expressed cytosolic heteropolymer composed of H-chains (FTH1) and L-chains (FTL) ... (Abcam, ab7475) to detect LC3B-FTH1 co-localization and with 10% normal donkey serum and 10% normal goat serum (Abcam, ab7481) to detect LAMP2a-FTH1 co-localization. The primary antibodies were rabbit anti-FTH1, …
Fth1 abcam
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WebSep 21, 2024 · FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis. Interestingly, FTH1 was also found to be involved in ferritinophagy, a … WebDec 4, 2024 · We noticed a huge increase in the expression of the ferroptosis marker ferritin heavy chain 1 (FTH1) in both clinical samples and mouse liver fibrosis. ... (Abcam, ab118970) according to the ...
WebJul 1, 2024 · The antibodies used were anti-GAPDH (Cell Signaling, 5174), anti-GPX4 (abcam, ab125066), anti-FTH1 (abcam, ab65080), anti-Transferrin (abcam, ab82411), ... FTH1 is the heavy subunit of ferritin, which plays an essential role in maintaining the balance of iron metabolism in cells. WebJun 3, 2024 · The primary antibodies used were anti-GPX4 antibody (Proteintech, Cat# 67763, 1:100), and anti-FTH1 antibody (Abcam, Cat# ab75973, 1:200). Lipid Peroxidation Staining. Cells were incubated for 30 min at 37°C with BODIPY™ 581/591C11 to detect lipid peroxidation. After incubation, the cells were harvested by trypsinization.
WebMar 5, 2024 · The major findings of the present study were as follows: (1) NSUN5 was downregulated in erastin-induced BMSC ferroptosis, while NSUN5 overexpression … WebNational Center for Biotechnology Information
WebMar 27, 2024 · The membranes were incubated overnight at 4 °C with the following primary antibodies: ACSL4 (Abcam), GPx4 (Abcam), FTH1 (Abcam), COX2 (Abcam), and β-actin (Sigma). Finally, the membranes were incubated with corresponding secondary antibodies for 90 min at room temperature, and densitometry measurements of the western blot …
WebFeb 20, 2024 · H-ferritin is not necessary for in vitro differentiation of bone marrow-derived macrophages. (a) Quantification of FTH1 and FTL by Western blot in protein extracts from Fth1 +/+ (black) and Fth1 ... lyrics walking up the kings highwayWebSupplementary Figure 3: Quality control of single-cell RNA-seq data and clustering of human chondrocytes.(A) Number of UMI, number of features, percent of mitochondrial gene and log10GenesPerUMI distribution of human chondrocytes in each of cartilage sample.(B) Visualize the correlation between the number of detected genes and the number of … kishigo premium black series parkaWebOct 25, 2024 · According to the instructions, the expression levels of ferritin FTL (ab155517, Abcam) and FTH1 (ab78877, Abcam) in the retina were detected using the corresponding kits. Immunohistochemistry (IHC) kishimoto interviewWebAbcam catalog: ab183781 total number of products: 13. domestic rabbit monoclonal (EPR18878) reactivity: human, mouse, rat application: western blot, immunohistochemistry - paraffin section ... The membrane was incubated with rabbit anti-FTH1 monoclonal antibody (Catalog # M02401) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 ... lyrics walking the wireWebOverview Product name Human FTH1 knockout HEK-293T cell line Parental Cell Line HEK293T Organism Human Mutation description Knockout achieved by using … lyrics walking to new orleansWebFeb 10, 2024 · Cell Signaling Technology's FTH1 (D1D4) Rabbit mAb is a Rabbit monoclonal antibody. The FTH1 (D1D4) Rabbit mAb Antibody was generated using FTH1 as the antigen. It reacts with Human, Mouse, Rat, and Non-Human Primate. This antibody has been shown to work in applications such as: Precipitation, Immunohistochemistry, … lyrics walkin on the sunWebDec 21, 2024 · PAS staining was also employed for morphologic evaluation using Periodic Acid Schiff Staining Kit (Abcam, catalog # ab150680). The sections were incubated with periodic acid solution for 10 min after de-paraffinization and rehydration. ... It is noteworthy that FTH1 was up-regulated in cisplatin-treated kidneys, unlike the changes in BUMPT ... lyrics walk in the light beautiful light