Phosphinothricin ppt

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WebApr 24, 2024 · Transaminase responsible for alienating prochiral ketone compound is applicable to asymmetric synthesis of herbicide L-phosphinothricin (L-PPT). In this work, the covalent immobilization of recombinant transaminase from Citrobacter koseri (CkTA) was investigated on different epoxy resins. WebApr 1, 2024 · Phosphinothricin (PPT) is one of the most prevalently using herbicides. The commercial phosphinothricin products are generally in the form of a racemic mixture, of …

菰抗病候选基因筛选及其转基因水稻白叶枯病抗性分析 - 豆丁网

WebApr 1, 2024 · L-phosphinothricin (L-PPT) is a competitive and environmentally friendly herbicide. To develop an efficient approach for synthesis of l-PPT, a kinetic resolution route with a novel... Web【技术实现步骤摘要】一种D ‑ 氨基酸氧化酶突变体及其应用： [0001]本专利技术涉及蛋白质工程领域，涉及一种D ‑ 氨基酸氧化酶突变体及其应用。技术介绍： [0002]草铵膦(glufosinate ammonium,phosphinothricin简称PPT)，化学名称为(D，L) ‑2‑ 氨基 ‑4‑ [羟基(甲基)膦酰基]丁酸。其分子式为：C5H 12 NO4P ... list of hotel kitchen equipment

Selection of BASTA resistant Arabidopsis transformants

WebJan 7, 2024 · In BY-2 cells, phosphinothricin had been already used by Nakamura et al. ( 2010 ), who recommended 2 mg/l PPT for selection. However, this protocol did not work in our hands, and later on, PPT was even considered unsuitable for suspension cultures by some authors (Seifertová et al. 2014 ). WebJan 12, 2024 · Phosphinothricin (PPT) is one of the most widely used herbicides. PTT targets glutamine synthetase (GS) activity in plants, and its phytotoxicity is ascribed to ammonium accumulation and reactive oxygen species bursts, which drives rapid lipid peroxidation of cell membranes. list of hotel five star in malaysia

The bar Gene as Selectable and Screenable Marker in Plant

WebA fatty acid photodecarboxylase (CvFAP) based photobiocatalytic deracemization of phosphinothricin (PPT) was developed. The engineered CvFAP enabled the kinetic … WebJan 1, 1995 · It consists of phosphinothricin (PPT), which is an irreversible inhibitor of glutamine synthetase (glutamate–ammonia ligase), and two l-alanine residues. Glufosinate ammonium, the chemically synthesized PPT, and bialaphos are used as … imaths foundationWebPhosphinothricin is a broad-spectrum herbicide that acts as a competitive inhibitor of glutamine synthetase. Glutamine synthetase plays an important role in plant nitrogen … list of hotel name

"WebJan 1, 1995 · The bialaphos resistance gene (bar) is part of the biosynthesis pathway of bialaphos 6.The strains Streptomyces hygroscopicus and Streptomyces … " - Phosphinothricin ppt

Phosphinothricin ppt

WebSep 15, 2014 · bar基因编码对除草剂PPT（Phosphinothricin）和双丙氨磷（Bialaphos）的抗性，适合用作禾谷类作物的转化体筛选。在进行候选克隆的功能互补转化过程中，用bar基因筛选较困难，假阳性率高。 Web丁香通为您提供cas85178605p商品详情介绍：价格：询价，货号：p839520-10mg，cas号：85178-60-5，品牌：进口，产地：进口，详见丁香通cas85178605p商品详情页；

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WebAug 16, 2007 · The present investigation describes a PPT-based selection system for alfalfa transformation that uses the phosphinothricin acetyl-transferase ( pat) gene as a selectable marker and 5.0 mg l −1 of bialaphos as the selective agent. WebOct 1, 1987 · Phosphinothricin (PPT) is a potent inhibitor of glutamine synthetase in plants and is used as a non-selective herbicide. The bar gene which confers resistance in Streptomyces hygroscopicus to ...

WebPhosphinothricin C5H11NO4P- CID 25244585 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, … WebPhosphinothricin (PPT) is the active ingredient in bialaphos, which specifically inhibits glutamine synthetase in land plants. We isolated a novel PPT-resistant gene from a soil …

WebSep 12, 2014 · 此可读框架通过组成型启动子CaMV35S启动，携带PPT乙酰基转移酶(PAT)基因作为选择标记基因，并将构建的表达载体导入根癌农杆菌EHA105。确定烟草转化的潮霉素临界筛选浓度为50mg，头孢噻肟钠的抑菌浓度为200mg。 WebPublished: October 1999 A facile method for screening for phosphinothricin (PPT)-resistant transgenic wheats Sonriza Rasco-Gaunt, Amanda Riley, Paul Lazzeri & Pilar Barcelo Molecular Breeding 5 , 255–262 ( 1999) Cite this article …

WebPhosphinothricin acetyltransferase ( EC 2.3.1.183, PAT, PPT acetyltransferase, Pt-N-acetyltransferase, ac-Pt) is an enzyme with systematic name acetyl-CoA:phosphinothricin …

WebWild-type strain 21gr of the green alga Chlamydomonas reinhardtii was resistant to the ammonium salt of l-phosphinothricin (PPT, also called glufosinate), an irreversible inhibitor of glutamine synthetase activity and the main active component of the herbicide BASTA (AgrEvo, Frankfurt am Main, Germany). Under the same conditions, however, this strain … imathservice.stubWebSep 12, 2024 · The transformed calli were selected on the media containing increasing concentrations of phosphinothricin (PPT, 3 mg/L and 6 mg/L). After shooting and rooting, the calli were cultured in the regeneration media for 2 weeks in light at 25 °C, and transferred to soil after 2-day adaption in water. list of hotel in klWebAbout 50 =B5M phosphinothricin (PPT, the active ingredient in Basta) is sufficient for the selection *** I used pure phosphinotricin for selection of BASTA resistant Arabidopsis seedlings harboring a p35S-bar gene. This worked nicely at a concentration of 0.05 mM. imaths foundation student bookWebJan 12, 2024 · Abstract. Phosphinothricin (PPT) is one of the most widely used herbicides. PTT targets glutamine synthetase (GS) activity in plants, and its phytotoxicity is ascribed … imaths box plotsWebDec 1, 1992 · Transgenic friable, embryogenic oat callus cultures were selected for phosphinothricin (PPT) resistance following microprojectile bombardment with a plasmid encoding the Streptomyces hygroscopicus ... list of hotel rewards programsWeb168 protocolsare often genotypespeciﬁc (Widholm, 1995; Somers et al., 2003). Increased soybean transforma-tion efﬁciency, may be achieved by further optimizing list of hotel room typesWebPost sterilization, seeds were spread out onto plates that contained standard Arabidopsis growth medium (half-strength Murashige and Skoog (MS) salts) which was supplemented with the selective agent, phosphinothricin (PPT), at a concentration of 10 mg/L. imathskids.com.au investigation 11